Feeding strategy optimization in interaction with target seeding density of a fed-batch process for monoclonal antibody production
نویسندگان
چکیده
Background Current trend towards Quality by Design (QbD) leads the process development exercise towards systematic experimentation, rational development, process understanding, characterization and control. In this study, an example of the application of QbD approach is given. Optimization of the feeding strategy and the target seeding density was performed and interactions of the two parameters were assessed in order to enhance cell growth and MAb productivity. The feeding strategy was optimized to take into account daily process performance attributes and associated nutrient needs of the culture to maintain a balance between metabolism and MAb productivity. For scale up the feed strategy was simplified to become independent of daily process performance attributes. Feed ranging studies were performed to assess the robustness of the process.
منابع مشابه
High cell density and high monoclonal antibody production through medium design and rational control in a bioreactor.
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